Luminescent polymer cyclic amplification
US8647889B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 14, 2012 |
| Grant date | Feb 11, 2014 |
| Priority date | — |
| Expiry date | Nov 14, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/757
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.