Methods for separating frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations
US8652769B2 · kind B2 · utility
1Cited by
491References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 9, 2010 |
| Grant date | Feb 18, 2014 |
| Priority date | — |
| Expiry date | Jun 22, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N1/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformily stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.