Mutant lactate oxidase with increased stability and product, methods and uses involving the same

US8652795B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Stefan Leitgeb · Graz, AT
• Thomas Meier · Cherbourg, FR
• Bernd Nidetzky · Graz, AT
• Bernhard Schaffar · Graz, AT
• Thomas Stoisser · Graz, AT

Key dates

• Filing date: Sep 18, 2012
• Grant date: Feb 18, 2014
• Expiry date: Sep 18, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/0069
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure relates to a mutant lactate oxidase having increased stability, a nucleic acid encoding the mutant lactate oxidase, an expression vector comprising the nucleic acid, a host cell comprising the nucleic acid or the expression vector, a method of determining lactate in a sample, the use of the mutant lactate oxidase for determining lactate, a device for determining lactate in a sample using the mutant lactate oxidase and a kit for determining lactate comprising the mutant lactate oxidase.