Patent · US Active

Marine bacterial strains of cycloclasticus and marinobacter for degradation of high-molecular-weight polycyclic aromatic hydrocarbons

US8652826B2 · kind B2 · utility

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5Claims

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Assignee

First Institute of Oceanography State Oceanic Administration of China · CN

Inventors

Zhi Cui · Dalian, CN
Li Zheng · Damaishan, CN
Guang-Su Xu · Damaishan, CN
Qian Li · Sydney, AU
Wei Gao · Laishui, CN
Fa-Rong Chen · Damaishan, CN

Key dates

Filing date	Feb 7, 2013
Grant date	Feb 18, 2014
Priority date	—
Expiry date	Feb 7, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC02F2103/08
WIPO fieldEnvironmental technology
WIPO sectorChemistry

Abstract

A composite marine bacterial liquid for the synergic degradation of high-molecular-weight polycyclic aromatic hydrocarbons (HMW PAHs) and the preparation method thereof. The composite marine bacterial liquid is generated by mixing the fermented culture liquid of a stain of Cycloclasticus sp. PY97M and a stain of Marinobacter sp. D15-8W, the cell concentration of each is 107-109 CFU/mL. The preparation method includes: inoculating the seed liquid of Cycloclasticus strains and Marinobacter strains individually into the sodium acetate medium or M8 medium, fermenting under the condition of defoaming by flowing addition of defoamer, and mixing the fermented cultures to obtain the composite marine bacterial liquid, then preparing a 5-fold concentrated liquid by centrifuging and storing at 4° C.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.