Methods for the cryopreservation of animal cells that contain high levels of intracellular lipids
US8652844B2 · kind B2 · utility
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15Claims
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Key dates
| Filing date | Jun 12, 2007 |
| Grant date | Feb 18, 2014 |
| Priority date | — |
| Expiry date | Nov 28, 2029 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/125
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A method for cryopreservation of animal cells with high level of intracellular lipid content, comprises the steps of conducting a delipation procedure using one or more lipolytic agent(s) and/or lipogenesis inhibitors during culture of the animal cells to stimulate the hydrolysis of intracellular lipids to reduce the lipid content, and vitrifying the treated animal cells using a modified vitrification solution and a modified warming solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.