Patent · US Active

Methods for purification of alpha-1-antitrypsin and apolipoprotein A-1

US8653245B2 · kind B2 · utility

3Cited by

1References

12Claims

0Family size

Assignee

CSL Behring AG · DE

Inventors

Nathan Brinkman · Herscher, US
Douglas Bigler · Kankakee, US
Reinhard Bolli · Worb, CH
Vreni Foertsch · Winznau, CH

Key dates

Filing date	Apr 5, 2013
Grant date	Feb 18, 2014
Priority date	—
Expiry date	Apr 5, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/8125
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

This invention relates to protein separation and purification methods for both alpha-1-antitrypsin (AAT, also known as alpha-1 proteinase inhibitor, API, and A.sub.1-PI) and Apolipoprotein A-I (ApoA-1) from, for example, a fraction of human blood plasma. In certain embodiments, the invention provides methods for separating AAT from ApoA-1 at the initial stage of purification, so that the same starting material can be used as a source for both proteins. The methods further pertain to providing compositions of AAT and of ApoA-1 suitable for pharmaceutical use and are suitable for large-scale purification.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.