Patent · US Active

Targeted DNA insertion in plants

US8653326B2 · kind B2 · utility

0Cited by

4References

13Claims

0Family size

Assignee

Bayer CropScience N.V. · BE

Inventors

Kathleen D′Halluin · Mariakerke, BE
Chantal Vanderstraeten · Evergem, BE
Rene Ruiter · Melle, BE

Key dates

Filing date	Jul 6, 2009
Grant date	Feb 18, 2014
Priority date	—
Expiry date	Oct 8, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/8213
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving “double stranded break” inducing enzymes. More specifically, the methods involve introducing the DNA that is to be inserted into the induced break into the plant cell via direct DNA delivery and incubating the plant cells in a plant phenolic compound prior to DNA delivery. Also provided are improved I-SceI encoding nucleotide sequences having a CG content of 50-60%.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.