Linear DNA molecule delivery using PEGylated quantum dots for stable transformation in plants

US8653327B2 · kind B2 · utility

Assignee

• Agrigenics Inc.

Inventors

• Narasimha C. Samboju · Carmel, US
• Kerrm Y. Yau · Carmel, US
• Frank Burroughs · Noblesville, US
• Jayakumar P. Samuel · Carmel, US
• Steven R. Webb · Westfield, US

Key dates

• Filing date: Jul 7, 2011
• Grant date: Feb 18, 2014
• Expiry date: Dec 11, 2031

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.