Patent · US Active

Use of a bis-maleic anhydride cross-linking agent for fixation of a cell or tissue sample

US8658109B2 · kind B2 · utility

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8Claims

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Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Hans-Peter Josel · Weilheim, DE
Michael Gerg · Munich, DE
Rupert Hermann · Weilheim, DE
Rosa Isabel Saez Diaz · Munich, DE

Key dates

Filing date	Feb 13, 2013
Grant date	Feb 25, 2014
Priority date	—
Expiry date	Feb 13, 2033

Classification

Technology area (CPC G)Physics
CPC primaryG01N1/30
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.