Determining liver toxicity of an agent using metabolite biomarkers
US8658351B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 5, 2010 |
| Grant date | Feb 25, 2014 |
| Priority date | — |
| Expiry date | Feb 5, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/17
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides various biomarkers for hepatotoxicity and various methods of using the biomarkers Some of the biomarkers within the scope of this invention are cholate, glycochenodeoxycholate, glycocholate, taurine, 3-hyroxy-2-ethylpropionate, 4-imidazoleacetate, tyramine, anthranilate, 2′-deoxycytidine, N-acetyl aspartate (NAA), beta-hydroxy-hexanoate, and sarcosine (N-methylglycine) The methods of using the biomarkers include exposing a first hepatocyte culture to a test agent and comparing the levels of the one or more biomarkers obtained in the first hepatocyte culture to the levels of the one or more biomarkers obtained in a second hepatocyte culture without the test agent, where differential levels of the one or more biomarkers in the first hepatocyte culture as compared to the levels in the second hepatocyte culture is indicative of the test agent being a hepatotoxicant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.