Ultra sensitive method for in situ detection of nucleic acids
US8658361B2 · kind B2 · utility
87Cited by
56References
60Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 21, 2011 |
| Grant date | Feb 25, 2014 |
| Priority date | — |
| Expiry date | Oct 21, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6841
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of an in situ hybridization (ISH) assay method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of the ISH assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.