Method for preparing high purity L-α glycerylphosphorylcholine

US8658401B2 · kind B2 · utility

Assignee

• Jiangnan University · CN

Inventors

• Yuanfa Liu · Wuxi, CN
• Kangyi Zhang · Hefei, CN
• Xingguo Wang · Fremont, US
• Xiangyun Qian · Changzhou City, CN
• Li Zhou · Toronto, CA
• Zhihua Song · Wuxi, CN

Key dates

• Filing date: Mar 24, 2011
• Grant date: Feb 25, 2014
• Expiry date: Nov 23, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/001
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed is a method for preparing L-α-Glycerylphosphorylcholine with high yields and purity. The method uses phospholipase A1-based enzymatic hydrolysis, ion-exchange resin purification and silica gel column chromatography to prepare L-α-glycerylphosphorylcholin with purity up to 99.8% and a final yield up to 78.4%. The method disclosed is simple, cost-effective, environmentally friendly, and adaptable to industrial applications.