Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids

US8658413B2 · kind B2 · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Melissa D. Bosak · Deptford, US
• Michael W. Bostick · Wilmington, US
• Hongxian He · Wilmington, US
• Seung-Pyo Hong · Hockessin, US
• Dana M. Walters Pollak · West Chester, US
• Pamela L. Sharpe · Newark, US
• Yehong Jamie Wang · Newark, US
• Zhixiong Xue · Chadds Ford, US
• Narendra S. Yadav · Wilmington, US
• Hongxiang Zhang · Chadds Ford, US
• Quinn Qun Zhu · West Chester, US

Key dates

• Filing date: Aug 26, 2011
• Grant date: Feb 25, 2014
• Expiry date: Nov 24, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y103/01035
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b5-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”], are also disclosed.