Whole proteome tiling microarrays

US8658572B2 · kind B2 · utility

Assignee

• ROCHE NIMBLEGEN, INC. · US

Inventors

• Tom Albert · Hamilton, US
• Todd Richmond · Fort Collins, US
• Matthew Rodesch · McFarland, US
• Klaus-Peter Stengele · Pleiskirchen, DE
• Jochen Bühler · Waldkraiburg, DE

Key dates

• Filing date: Mar 13, 2012
• Grant date: Feb 25, 2014
• Expiry date: Mar 13, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2570/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to a microarray comprising at least 50,000 oligopeptide features per cm2 where the oligopeptide features represent at least 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100% of the proteome of a virus or an organism. The present invention further relates to methods for the synthesis of such microarrays and methods of using microarrays comprising at least 50,000 oligopeptide features per cm2. In an embodiment of the invention, the oligopeptide features represent proteins expressed in the same species, wherein the oligopeptide features are presented in a tiling pattern representing at least about 5,000 to-at least about 25,000 proteins expressed in a species. In some embodiments, the oligopeptide microarray features represent proteins expressed in the same species, wherein the microarray features are present in a tiling pattern that represents at least about 5,000 to at least about 50,000 expressed proteins.