Method for the detection of post-translational modifications
US8663928B2 · kind B2 · utility
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Key dates
| Filing date | Nov 27, 2007 |
| Grant date | Mar 4, 2014 |
| Priority date | — |
| Expiry date | Jul 23, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/485
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method for the detection in homogeneous medium of a post-translational modification of a proteinaceous substrate catalyzed by a cell enzyme, characterized in that the post-translational modification reaction takes place in intact living cells, in that these cells comprise a heterologous expression vector coding for a fusion protein comprising the proteinaceous substrate and a first coupling domain and in that it comprises the following stages:
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