Multicolor flow cytometry compositions containing unconjugated phycobiliproteins
US8663935B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Dec 10, 2009 |
| Grant date | Mar 4, 2014 |
| Priority date | — |
| Expiry date | Jan 7, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/533
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Novel compositions containing (i) ligand conjugated phycobiliprotein tandem dyes and (ii) unconjugated phycobiliproteins are provided wherein the phycobiliproteins are derived from the same bacterial or eukaryotic algae species. The phycobiliproteins must be the same or different, but must contain some non-crosslinked subunits which exchange. Also provided are methods for preparing these compositions, kits containing these compositions or components of the same, and methods of using these compositions for cellular and non-cellular analysis.
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