Protein glycosylation modification in methylotrophic yeast
US8663971B2 · kind B2 · utility
12Cited by
6References
12Claims
0Family size
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Key dates
| Filing date | Apr 16, 2009 |
| Grant date | Mar 4, 2014 |
| Priority date | — |
| Expiry date | Mar 30, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/52
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an α-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.