Retroviral vector particles and methods for their generation and use
US8663989B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 22, 2010 |
| Grant date | Mar 4, 2014 |
| Priority date | — |
| Expiry date | Nov 22, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/203
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods of host cell transduction utilizing ecotropic retroviral vector particles. The retroviral vector particle may comprise an envelope of Friend murine leukaemia virus, in particular the envelope encoded by molecular clone PVC-211 and the host cell may be engineered to recombinantly express the Reel receptor. The retroviral vector particles and methods of the invention can be used to introduce expressible polynucleotide sequences of interest into host cells with high efficiency. This results in protein production methods with higher yield (mg/L) and a reduction in manufacturing costs that could be used in a range of applications including for example, the production of therapeutic proteins, vaccines and antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.