Patent · US Active

Automated protein analyzer

US8663993B2 · kind B2 · utility

0Cited by
21References
15Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 11, 2010
Grant dateMar 4, 2014
Priority date
Expiry dateMay 5, 2031

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/25
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A dye binding method for protein analysis is disclosed. The method includes the steps of preparing an initial reference dye solution of unknown concentration from an initial reference dye concentrate and creating an electronic signal based upon the absorbance of the initial reference dye solution. Thereafter, an electronic signal is created based upon the absorbance of a dye filtrate solution prepared from the initial reference dye solution and an initial protein sample. The absorbance signals from the reference dye solution and the dye filtrate solution are sent to a processor that compares the respective absorbances and calculates the protein content of the protein sample based upon the difference between the absorbances. An electronic signal is created based upon the absorbance of a successive dye filtrate solution prepared from the reference dye solution and a successive protein sample, and the absorbance signal from the successive sample dye filtrate solution is sent to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate s…

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.