Patent · US Active

Probes for specific analysis of nucleic acids

US8664164B2 · kind B2 · utility

62Cited by

1References

18Claims

0Family size

Assignee

AGILENT TECHNOLOGIES, INC. · US

Inventors

Olof John Ericsson · Uppsala, SE
Magnus Isaksson · Täby, SE
Henrik Johansson · Uppsala, SE
Ulf Landegren · Uppsala, SE

Key dates

Filing date	Jul 23, 2010
Grant date	Mar 4, 2014
Priority date	—
Expiry date	Jul 23, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6855
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides a method for detecting or enriching for a target deoxyribonucleic acid (DNA) present in a nucleic acid sample, said method comprising: (a) fragmenting a nucleic acid sample to generate nucleic acid fragments including a target fragment containing said target DNA and non-specifically ligating an adaptor sequence to an end of said fragments; (b) rendering said fragments at least partially single-stranded; (c) contacting the at least partially single-stranded fragments of step (b) with oligonucleotides A and B of a single target-specific nucleic acid probe; (d) ligating oligonucleotide B of said probe to the part of the single-stranded portion of said target fragment which is hybridised to oligonucleotide A of said probe to produce a probe-target fragment hybrid; and (e) detecting or enriching for said probe-target fragment hybrid.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.