Predictive model for use in sequencing-by-synthesis
US8666678B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 27, 2011 |
| Grant date | Mar 4, 2014 |
| Priority date | — |
| Expiry date | Apr 2, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B5/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method of obtaining a more accurate estimate of a signal correction parameter(s) in sequencing-by-synthesis operations, such as incomplete extension rates, carry forward rates, and/or signal droop rates. The sequencing operation produces signal data. A model is constructed to simulate a population of template strands as it undergoes the sequencing process and becomes divided into different phase-states as the sequencing-by-synthesis progresses. For example, the model may be a phase-state model. The output from the model is used to adjust the signal correction parameter(s). For example, the model may be fitted to the signal data. This fitting results in a more accurate estimate of the signal correction parameter(s). In another embodiment, the signal droop rate is modeled as a decaying function and this decaying function is fitted to the signal data to obtain an improved estimate of the signal droop rate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.