Transformation of gram positive bacteria by sonoporation
US8669085B2 · kind B2 · utility
1Cited by
2References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 29, 2010 |
| Grant date | Mar 11, 2014 |
| Priority date | — |
| Expiry date | Aug 27, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N13/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.