Patent · US Active

Methods of producing proteins in Chinese hamster ovary (CHO) cells

US8669109B2 · kind B2 · utility

0Cited by

2References

21Claims

0Family size

Assignee

BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG · DE

Inventors

Ralf Otto · Wiesbaden, DE
Barbara Enenkel · Warthausen, DE
Juergen Fieder · Unterstadion, DE
Thomas Krieg · Erbach, DE

Key dates

Filing date	Oct 7, 2010
Grant date	Mar 11, 2014
Priority date	—
Expiry date	Oct 7, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2510/02
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.