Methods and compositions for increasing plant transformation efficiency
US8669417B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 20, 2010 |
| Grant date | Mar 11, 2014 |
| Priority date | — |
| Expiry date | Feb 25, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8281
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods and compositions for increasing the efficiency of genetic transformation of host cells, including plant cells, and other eukaryotic cells, by reducing the expression of a polypeptide active in a pathway, such as the NHEJ pathway, for repairing damage to the cellular genome. In certain embodiments, the polypeptide is active in repairing double strand breaks (DSB's) of a cellular genome, and may include XRCC4, KU70, KU80, the DNA-activated Protein Kinase (DNA-Pkcs), and ATM. Methods for enhancing the resistance of plant cells to Crown Gall disease are also provided. In another aspect, genetic regulatory elements are provided, including an XRCC4 promoter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.