Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions

US8673318B2 · kind B2 · utility

Assignee

• Bavarian Nordic A/S · DK

Inventors

• Ingmar Rathe · Olching, DE
• Eva Felder · Munich, DE
• Karl Heller · Unterföhring, DE

Key dates

• Filing date: Nov 29, 2012
• Grant date: Mar 18, 2014
• Expiry date: Nov 29, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/24151
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.