Methods, plasmid vectors and primers for assessing HIV viral fitness

US8673551B2 · kind B2 · utility

Assignees

• SPEEDX PTY LTD · AU
• Janssen R & D Ireland · IE

Inventors

• Laurence Tatiana Rimsky · Kapellen, BE
• Inky Paul Madeleine De Baere · Temse, BE
• Bart Anna Julien Maes · Hoboken, BE
• Marie-Pierre De Bethune · Everberg, BE
• Guenter Kraus · Sint-Katelijne-Waver, BE
• Elisa Mokany · Sydney, AU
• Alison Velyian Todd · Sydney, AU

Key dates

• Filing date: Dec 7, 2006
• Grant date: Mar 18, 2014
• Expiry date: Jan 15, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/703
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to methods and means for the evaluation of HIV replicative capacity in a given environment. In particular, the invention provides a growth competition assay that can determine relative viral fitness using a recombinant tagged HIV-1 virus system. The methods rely on plasmid vectors, amplicons, primers and probes, and the generation of replication-competent viruses therefrom. Said methods and materials may find use in multiple fields including diagnostics, drug screening, pharmacogenetics and drug development.