Protein expression system
US8674084B2 · kind B2 · utility
10Cited by
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25Claims
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Key dates
| Filing date | Jan 8, 2009 |
| Grant date | Mar 18, 2014 |
| Priority date | — |
| Expiry date | Mar 21, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8258
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The inventions is based on an expression enhancer sequence derived from the RNA-2 genome segment of a bipartite RNA virus, in which a target initiation site in the RNA-2 genome segment has been mutated. Deletion of appropriate start codons upstream of the main RNA2 translation initiation can greatly increase in foreign protein accumulation without the need for viral replication. Also provided are methods, vectors and systems, including the ‘hyper-translatable’ Cowpea Mosaic Virus (‘CPMV-HT’) based protein expression system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.