Modifying the DNA recombination potential in eukaryotes
US8674179B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 2011 |
| Grant date | Mar 18, 2014 |
| Priority date | — |
| Expiry date | May 24, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/102
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods of modifying in vivo mutagenesis or homologous and homeologous recombination in a eukaryote. The method of modifying in vivo mutagenesis involves transforming a eukaryote with a nucleotide sequence capable of expressing a wild-type prokaryotic MutS, MutL, MutH, MutU, NLS-MutS, NLS-MutL, NLS-MutH, NLS-MutU protein, or a combination thereof, and expressing the protein. A method of modifying recombination between homologous chromosomes in an allopolyploid eukaryotic organism comprising, expressing a nucleotide sequence encoding prokaryotic NLS-MutS in combination with one or more than one of NLS-MutL, NLS-MutH, or NLS-MutU, within a germ cell of the allopolyploid eukaryotic organism is also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.