Covalent immobilization of molecules comprising an amino group

US8679807B2 · kind B2 · utility

Assignee

• Lab-On-A-Bead · SE

Inventors

• Sven Oscarsson · Örbyhus, SE
• Kristofer Eriksson · Hagfors, SE
• Leif Nyholm · Uppsala, SE

Key dates

• Filing date: Jul 27, 2011
• Grant date: Mar 25, 2014
• Expiry date: Jul 27, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54353
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

There is provided a method for covalent immobilization of at least one molecule comprising at least one amino group, said method comprising the sequential steps of: a) providing a surface comprising —SH groups, b) oxidizing the surface comprising —SH groups using redox reactions in the presence of noble metal ions, and c) contacting the surface with at least one molecule comprising at least one amino group to obtain a covalent binding of the at least one molecule to the surface, wherein said at least one amino group is involved in obtaining said covalent bond. The immobilized molecules are immobilized via stable covalent bonds. The method is more versatile since it can be performed as a one step method. All reaction steps are performed in aqueous solution. All steps can be performed at room temperature. The chemicals used are less expensive and less toxic compared to the prior art.