Cell-matrix microspheres, methods for preparation and applications

US8679809B2 · kind B2 · utility

Assignee

• University of Hong Kong · CN

Inventors

• Barbara Pui Chan · Hong Kong, CN
• Godfrey Chi-Fung Chan · Hong Kong, CN
• Hoi Ling Wong · Sha Tin, CN
• Pik To Cheung · Hong Kong, CN
• Song-Eng Kathryn Cheah · Hong Kong, CN
• Danny Chan · Fremont, US

Key dates

• Filing date: May 18, 2007
• Grant date: Mar 25, 2014
• Expiry date: Dec 1, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/90
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method has been developed to produce stable cell-matrix microspheres with up to 100% encapsulation efficiency and high cell viability, using matrix or biomaterial systems with poor shape and mechanical stability for applications including cell therapeutics via microinjection or surgical implantation, 3D culture for in vitro expansion without repeated cell splitting using enzymatic digestion or mechanical dissociation and for enhanced production of therapeutic biomolecules, and in vitro modeling for morphogenesis studies. The modified droplet generation method is simple and scalable and enables the production of cell-matrix microspheres when the matrix or biomaterial system used has low concentration, with slow phase transition, with poor shape and mechanical stability.