Testing method of nucleic acid binding protein based on biochip

US8680016B2 · kind B2 · utility

Assignees

• CAPITALBIO CORPORATION · CN
• TSINGHUA UNIVERSITY · CN

Inventors

• Wei Shao · Frederick, US
• Yongchao Zhao · Lo Wu, CN
• Yimin Sun · Beijing, CN
• Jiying Qiao · Beijing, CN
• Huajiang Wei · Beijing, CN
• Weiping Yang · San Diego, US
• Yuxiang Zhou · Beijing, CN
• Jing Cheng · Beijing, CN

Key dates

• Filing date: Nov 23, 2004
• Grant date: Mar 25, 2014
• Expiry date: Nov 11, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6837
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A testing method of nucleic acid binding protein based on biochip, comprises the following steps: 1. puts a plurality of groups solution including nucleic acid captured probes into biological sample including a plurality of nucleic acid binding protein to be test, and thus forming nucleic acid captured probe-nucleic acid binding protein complexes; such nucleic acid captured probe includes at least a segment of binding sequence which can bind with aimed nucleic acid binding protein; 2. separates such nucleic acid captured probe-nucleic acid binding protein complexes, then recoveries nucleic acid captured probes; 3. hybridizes the nucleic acid captured probes according to step 2 with a plurality of single strand blotting probes on biochip substrate; the sequence of such blotting probe compensates with such nucleic acid captured probe or one of its strand; 4. detects the result of hybridization.