Patent · US Active

Use of microRNAs to control virus helper nucleic acids

US8680258B2 · kind B2 · utility

10Cited by

22References

31Claims

0Family size

Assignee

ALPHAVAX, INC. · US

Inventors

Vernon McNeil Coffield, III · Raleigh, US
Kurt Iver Kamrud · Apex, US
Jonathan F. Smith · Cary, US

Key dates

Filing date	Nov 25, 2009
Grant date	Mar 25, 2014
Priority date	—
Expiry date	Nov 25, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2820/60
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Provided herein are helper nucleic acids comprising at least one microRNA target sequence of an endogenous, cellular microRNA and a nucleic acid encoding a viral protein, wherein the microRNA target sequence is located in the untranslated or translated region of the nucleic acid encoding the viral protein. Also provided are vector systems, compositions and cells comprising the provided helper nucleic acids and a vector or replicon. Methods of making virus-like replicon particles and populations of virus-like replicon particles (VRP) are also provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.