Field flattening correction method for fluorescence imaging system
US8681247B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 12, 2011 |
| Grant date | Mar 25, 2014 |
| Priority date | — |
| Expiry date | Jul 26, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6493
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Systems and methods for flattening the image across the entire field by correcting the image for both the fluorescence and scatter spatial variations. Images of a stable fluorescence target and a scattering target are separately acquired in an imaging system. From these target images, a pixel remapping function, e.g., including two correcting pixel slopes, is calculated for subsequent image pixel remapping. An image of a sample under investigation is then acquired by the imaging system and the sample image is remapped based on the pixel remapping function for the imaging system to form a corrected (field flattened) image. Which correction pixel slope to be used is determined based on whether a sample image pixel value is higher or lower than a threshold value.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.