VCP-based vectors for algal cell transformation
US8685723B2 · kind B2 · utility
5Cited by
35References
6Claims
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Key dates
| Filing date | Nov 26, 2012 |
| Grant date | Apr 1, 2014 |
| Priority date | — |
| Expiry date | Nov 26, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8247
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are exemplary vectors for transforming algal cells. In exemplary embodiments, the vector comprises a Violaxanthin-chlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell. Embodiments of the invention may be used to introduce a gene (or genes) into the alga Nannochloropsis, such that the gene(s) are expressed and functional. This unprecedented ability to transform Nannochloropsis with high efficiency makes possible new developments in phycology, aquaculture and biofuels applications.
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