Cell sorting system and methods

US8691164B2 · kind B2 · utility

Assignee

• Celula, Inc. · US

Inventors

• William Butler · San Ramon, US
• Haichuan Zhang · San Diego, US
• Philippe Marchand · Lake Forest, US
• Keunho Ahn · San Diego, US
• Yi Zhang · Tuji, CN
• John Francis · San Diego, US
• Benjamin Lai · San Diego, US
• Eugene Tu · San Diego, US

Key dates

• Filing date: Jul 23, 2007
• Grant date: Apr 8, 2014
• Expiry date: Jun 1, 2030

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/2575
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on a switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format. Optical and/or fluidic switching forces are used alone or in combination to effect switching.