Method for detecting variations in nucleic acid sequences
US8691504B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 26, 2010 |
| Grant date | Apr 8, 2014 |
| Priority date | — |
| Expiry date | May 26, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method and a kit for detecting nucleic acid sequence variation using melting curve analysis, especially relates to a method and a kit for detecting nucleic acid sequence variation by melting curve analysis using self-quenched probe. Said method provides the characteristics of the self-quenched probe employed, as well as the corresponding nucleic acid amplification conditions, so that the probe can bind to the amplified target sequence, and variations of the target sequence can be detected by melting curve analysis. The present invention also encompasses a kit assembled according to the method described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.