Multi-primer amplification method for barcoding of target nucleic acids
US8691509B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 2, 2010 |
| Grant date | Apr 8, 2014 |
| Priority date | — |
| Expiry date | Jul 19, 2031 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0655
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
In certain embodiments, the invention provides amplification methods in which nucleotide tag(s) and a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.