Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same
US8691547B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 27, 2006 |
| Grant date | Apr 8, 2014 |
| Priority date | — |
| Expiry date | Dec 27, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/904
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.