Method and kit for examination of cells using N-(9-acridinyl) maleimide (NAM) or using 7-diethylamino-3-((4′-iodacetyl)amino)phenyl)-4-methylkcoumarin (CPI)
US8697390B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 14, 2009 |
| Grant date | Apr 15, 2014 |
| Priority date | — |
| Expiry date | Jul 14, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/502
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells. The present invention relates to the use of N-(9-acridinyl)maleimide (NAM) or to the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species (e.g., sulphur-containing species) present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead) cells. The present invention also relates to the use of NAM or to the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of NAM or with the use of 7-diethylamino-3-((4′-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI). The invention further relates to compositions used in methods for analyzing cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.