Compositions for the in vitro derivation and culture of embryonic stem (ES) cell lines with germline transmission capability and for the culture of adult stem cells
US8697444B2 · kind B2 · utility
11Cited by
9References
11Claims
0Family size
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Key dates
| Filing date | Nov 20, 2008 |
| Grant date | Apr 15, 2014 |
| Priority date | — |
| Expiry date | Nov 29, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2502/99
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention encompasses compositions for deriving, maintaining, and growing pluripotent and germ-line competent mammalian embryonic stem cells, and for deriving, maintaining, and growing adult human stem cells and/or adult early progenitor cells. Such compositions comprising a conditioned medium of a cell line expressing limited amounts of Leukemia Inhibitory Factor (LIF). The media of the present invention are used or for the generation of pluripotent and germ-line competent embryonic stem cells of mammals, and for the generation of adult human stem cells and/or adult early progenitor cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.