Ligand binding stabilization method for drug target identification

US8703438B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Jing Huang · New Taipei, TW
• Brett E. Lomenick · Los Angeles, US
• Rui Hao · Shandong, CN
• Nao Jonai · Yokohama, JP
• Thomas M. Vondriska · Los Angeles, US
• Sarah Warburton · Los Angeles, US
• Gregory Joseph Baker · Los Angeles, US
• Mariam Aghajan · Los Angeles, US

Key dates

• Filing date: Nov 18, 2009
• Grant date: Apr 22, 2014
• Expiry date: Oct 29, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/04
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Drug affinity responsive target stability (DARTS) is a method of drug target ID with several significant advantages over current techniques. In certain embodiments the method involves contacting a sample comprising one or more protein target(s) with a test agent to form a sample/test agent mixture; contacting the mixture with a protease; and identifying a protein or protein fragment that is protected from proteolysis, wherein the protection from proteolysis is an indicator that the protein or protein fragment binds to or interacts with the test agent.