Molecular diagnostics amplification system and methods
US8703445B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 15, 2006 |
| Grant date | Apr 22, 2014 |
| Priority date | — |
| Expiry date | Jul 25, 2028 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0481
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to automated devices and methods for the amplification of segments of nucleic acid in a convenient and portable manner. A single-use nucleic acid amplification device for producing an amplicon includes a housing and an amplification chamber. The chamber includes an ingress with a first reversible seal, an egress with a second reversible seal, a sealable sample entry orifice, and a first wall forming a portion of the chamber. The first wall includes a thermally conductive material and includes an interior surface and an exterior surface. The exterior surface includes a heating circuit and a temperature sensor. The sample entry orifice permits a sample of nucleic acid to enter the amplification chamber. The ingress is connected to a first conduit along with a pneumatic pump and a fluid pouch. The egress is connected to a second conduit permitting egress of the amplicon from the amplification chamber.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.