Baculoviruses with enhanced virion production and a method for the production of baculoviruses
US8703469B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 13, 2011 |
| Grant date | Apr 22, 2014 |
| Priority date | — |
| Expiry date | Sep 13, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2810/6081
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of co-expressing a portion of the VSV G protein gene or a truncated “stem” portion with GP64 and a retrovirus increases the titer of retroviral vectors. A truncated VSV G protein, preferably comprised of a small segment from the C-terminal portion of the ectodomain plus the transmembrane (TM) and cytoplasmic tail (CTD) domains of VSV G, co-expressed with retroviral vectors, enhances the production titers of the retroviral vectors. A preferred embodiment uses a VSV G construct that includes an N-terminal c-Myc epitope plus 42 amino acids from the C-terminal portion of the ectodomain, 20 amino acids from the predicted TM domain, and 29 amino acids from the predicted CTD of the VSV G protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.