Methods for detecting DNA-binding proteins
US8709722B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 24, 2012 |
| Grant date | Apr 29, 2014 |
| Priority date | — |
| Expiry date | Aug 24, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/922
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
There is provided a method for detecting binding of a DNA-binding protein to a target recognition sequence. The method comprises mixing in a reaction buffer a first set of metal nanoparticles, a second set of metal nanoparticles and a DNA-binding protein to form a mixture, and detecting the aggregation state of the mixture of metal nanoparticles. Each set of metal nanoparticles has a conjugated double-stranded DNA molecule having a single-stranded overhang at one end. The single-stranded overhangs of each set of DNA-conjugated metal nanoparticles are complementary to each other such that annealing of the complementary overhangs results in formation of the target recognition sequence that specifically binds the DNA-binding protein. The reaction buffer comprises an ionic species in a concentration sufficient to result in aggregation of the metal nanoparticles upon annealing of the first and second single-stranded overhang.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.