Use of endogenous promoters in genetic engineering of Nannochloropsis gaditana
US8709766B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 17, 2012 |
| Grant date | Apr 29, 2014 |
| Priority date | — |
| Expiry date | Oct 17, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/12
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure is directed to novel polynucleotide sequences for use in Nannochloropsis gaditana. The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs wherein N. gaditana promoters/control regions are operatively linked to N. gaditana or non-N. gaditana coding sequences. These novel polynucleotide sequences and expression constructs can be introduced into N. gaditana and can recombine into the N. gaditana genome. Expression from these polynucleotide sequences and expression constructs can enhance N. gaditana biomass and/or lipid biosynthesis. Also disclosed are methods for modifying N. gaditana, for example by stably transforming N. gaditana with nucleic acid sequences, growing the modified N. gaditana, and obtaining biomass and biofuels from the modified N. gaditana.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.