Process for the enzymatic reduction of enoates

US8709767B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Stephan Maurer · Neustadt, DE
• Bernhard Hauer · Neheim, DE
• Melanie Bonnekessel · Mannheim, DE
• Kurt Faber
• Clemens Stückler · Graz, AT

Key dates

• Filing date: Oct 23, 2012
• Grant date: Apr 29, 2014
• Expiry date: Oct 23, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P7/22
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process for the enzymatic reduction of an enoate (1) wherein the C═C bond of the enoate (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate (2) in a system which is free of NAD(P)H,in which A is a ketone radical (—CRO), an aldehyde radical (—CHO), a carboxyl radical (—COOR), with R═H or optionally substituted C1-C6-alkyl radical, R1, R2 and R3 are independently of one another H, —O—C1-C6-alkyl, —O—W with W=a hydroxyl protecting group, C1-C6-alkyl, which can be substituted, C2-C6-alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or one of R1, R2 and R3 is a —OH radical, or R1 is linked to R3 so as to become part of a 4-8-membered cycle, or R1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R1, R2 and R3 may not be identical.