Method of isolating human neuroepithelial precursor cells from human fetal tissue
US8709807B2 · kind B2 · utility
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Key dates
| Filing date | Mar 30, 2012 |
| Grant date | Apr 29, 2014 |
| Priority date | — |
| Expiry date | Mar 30, 2032 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K35/12
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for isolating human neuroepithelial precursor cells from human fetal tissue by culturing the human fetal cells in fibroblast growth factor and chick embryo extract and immunodepleting from the cultured human fetal cells any cells expressing A2B5, NG2 and eNCAM is provided. In addition, methods for transplanting these cells into an animal are provided. Animals models transplanted with these human neuroepithelial precursor cells and methods for monitoring survival, proliferation, differentiation and migration of the cells in the animal model via detection of human specific markers are also provided.
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