Patent · US Active

Potentiometric-sensor chip, potentiometric assay, and assay kit

US8712701B2 · kind B2 · utility

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1References
10Claims
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Key dates

Filing dateOct 29, 2009
Grant dateApr 29, 2014
Priority date
Expiry dateOct 12, 2030

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N33/49
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Provided is a potentiometric sensor chip in which the positional relationship among a reference electrode, a measurement electrode, and a sample inlet which enables measurement from the start of a reaction is defined, and further provided is a method for detecting the start time of the reaction. A very small amount of sample is measured with high accuracy. The very small quantity of sample is measured by a rate assay. When a reference electrode (103) is disposed between a sample inlet (102) and a measurement electrode (104), a sample solution arrives at the reference electrode (103) earlier than at the measurement electrode (104), whereby the surface potential of the measurement electrode (104) can be measured simultaneously when the sample solution arrives at the measurement electrode (104) and dissolves a reagent and thereby a reaction starts. The arrival of the sample solution at the measurement electrode (104) can be sensed by detecting the discontinuous change of the voltage observed by a voltmeter (106) at this time.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.