Protein scaffolds and viral particles for detecting analytes
US8715923B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 1, 2005 |
| Grant date | May 6, 2014 |
| Priority date | — |
| Expiry date | Sep 13, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/18022
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to compositions and methods for detecting analytes using detectably labeled fluorescent protein scaffolds. In certain embodiments of the invention, the scaffolds are viral particles in which the capsid viral structure provides a scaffold to attach detectably labeled fluorescent dyes and capture moieties that can be utilized to determine the presence of a desired analyte in a sample using any suitable method. The protein scaffold can contain amino acids carrying reactive groups (e.g., amines and thiols) that are spatially distributed on it with large enough separation to enable the attachment of a greater number of fluorescent label molecules without quenching.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.