Preparation of templates for nucleic acid sequencing
US8715966B2 · kind B2 · utility
12Cited by
3References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 19, 2012 |
| Grant date | May 6, 2014 |
| Priority date | — |
| Expiry date | Apr 19, 2032 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00722
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.